Our extensive experience in gene synthesis technologies has afforded us the capability to take on the most complex of gene sequences. Unlike traditional molecular cloning technologies, Biomatik’s gene synthesis service offers cost-effective solutions and quick turn-around time for your custom DNA synthesis, cloning, and/or recombinant expression project needs. We also offer a wide range of downstream services, including subcloning, mutagenesis, and plasmid preparations.
Select publications featuring custom genes synthesized by us:
Nature. 2020 Mar 5;180(5):878-894.e19. doi: 10.1016/j.cell.2020.01.029. Epub 2020 Feb 13. PMID: 32059783
Title: Lymphoma Driver Mutations in the Pathogenic Evolution of an Iconic Human Autoantibody
Science. 2019 Apr 12;364(6436). pii: eaav0748. doi: 10.1126/science.aav0748. PMID: 30975858
Title: Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides
Gene Synthesis Service Highlights
Over 98% success rate with "No Gene, No Charge" policy.
Free codon optimization upon request.
Free subcloning into one of our in-stock vectors - 120+ vectors to choose from!
Fast turnaround time with 100% sequence accuracy guarantee.
Site directed mutagenesis service .
One-stop for custom gene, peptide, protein, and antibody services.
Deliverables, Turnaround Time and Pricing
4 µg of lyophilized plasmid containing the sequence verified gene insert.
QC data includes: project QA report, complete construct map, and sequence chromatogram.
Gene Synthesis Standard Workflow
There are 6 basic steps involved in generating a synthetic gene sequence:
1. Chemical synthesis of short oligonucleotides.
2. Oligonucleotides annealing by ligation or polymerase reactions.
3. Cloning of gene sequences into specific vectors.
4. Screening of E. coli clones to identify putative candidates.
5. Sequencing to verify correct plasmid sequence.
6. Lyophilization of purified plasmid.