DNA Synthesis
Helix provides a very flexible and abundant primer customization scheme. By reading this description. you can easily customize primer products to meet your application needs. And we can give you specialized guidance timely.
Standard Custom Oligos
Comparison Table Of Synthetic Specifications And Final Yield(15-60nt）
| Purification Method | Synscale | Final Yield |
|---|---|---|
| OPC | 50nmol | 2OD |
| 100nmol | 5OD | |
| 200nmol | 10OD | |
| PAGE | 50nmol | 1OD |
| 100nmol | 2OD | |
| 200nmol | 3OD | |
| HPLC | 50nmol | 1OD |
| 100nmol | 2OD | |
| 200nmol | 5OD |
Different Purification Options And Primer Length
1- Introduction and price list of OPC purification primers
Purification with reverse-phase silica gel column which can effectively remove salts. The purity can meet the needs of most molecular biology experiments.
Advantages
● Fast
● High throughput
● Cost-effective
Applications
PCR amplification, gene synthesis, DNA sequencing, NGS capture probes, NGS Multiplex PCR oligos.
| Primer length | purification method | yield |
|---|---|---|
| 15-60nt | OPC | 2OD |
| 15-60nt | OPC | 5OD |
| 15-60nt | OPC | 10OD |
| 15-60nt | OPC | ＞10OD |
2. Introduction PAGE purification primers
Using denaturing polyacrylamide gel electrophoresis, oligo of different molecular weights migrate at different speeds during electrophoresis, allowing for separation. Finally, the target DNA is recovered from the gel.
Advantages
● Purity can be > 90%
● Effective for purification of long-stranded oligo
Applications
Mutation libraries, protein binding gel migration electrophoresis analysis, modified oligos such as phosphate and biotin.
| Primer Length | purification method | yield |
|---|---|---|
| 15-60nt | PAGE | 2OD |
| 15-60nt | PAGE | 5OD |
| 15-60nt | PAGE | 10OD |
| 15-60nt | PAGE | ＞10OD |
| * Markup prices are PAGE purification fees only | ||
3.Introduction HPLC purification primers
The purification of oligo with HPLC can achieve high purity and is mainly used for short chain and modified oligo.
Advantages
● Purity can reach > 90%
● Remove N-1 short fragment effectively
● Purification for modified oligo with hydrophobic groups
Applications
Point mutation, subcloning, diagnostic PCR protein binding gel migration electrophoresis analysis, modified primers with hydrophobic groups.
| Primer Length | purification method | yield |
|---|---|---|
| 15-60nt | HPLC | 2OD |
| 15-60nt | HPLC | 5OD |
| 15-60nt | HPLC | 10OD |
| 15-60nt | HPLC | ＞10OD |
| 61-90nt | HPLC | 2OD |
| 61-90nt | HPLC | 5OD |
| 61-90nt | HPLC | 10OD |
| 61-90nt | HPLC | >10OD |
| 90-130nt | HPLC | 1OD |
| 90-130nt | HPLC | 2OD |
| 90-130nt | HPLC | 5OD |
| 90-130nt | HPLC | >5OD |
| * Markup prices are HPLC purification fees only | ||