Description
1The amaR OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. The amaR OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, and fluorescence dye. The amaR OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonucle ase activity. The amaR OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. The amaR OnePCR™ contents red tracking dyes, provide a safe, non-toxic and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that are environmentally friendly containing no hazardous chemicals. The tracking dyes that run at 10 bp on a 1% agarose gel. The amaR OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for t he addition of primer and template solutions. The reagents are provided with the sufficient amplification reactions of 20 μl each.
amaR OnePCR reagent stability test after 3 months under different storage conditions. (Exposure to blue light)
– Lane M, marker (DM010-R500)
– Lane 1(1K), RT for 3 months
– Lane 2(1K), 4°C for 3 months
– Lane 3(1K), -20°C for 3 months
– Lane 4(2K), RT for 3 months
– Lane 5(2K), 4°C for 3 months
– Lane 6(2K), -20°C for 3 months