Specification:

• Principle : mini spin column (silica matrix)
• Sample size :1. Wet weight ≦ 100 mg
  2. Dry weight ≦ 20 mg
  
• Operation time : 30 ~ 60 minutes
• Binding capacity : up to 60 μg total DNA / column
• Expected yield : 5 ~ 40 μg /prep
• Column applicability : centrifugation and vaccum
• Minimum elution volume : 50 μl

Important Notes:

• Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
• Check PG1 Buffer before use, Warm PG1 Buffer at 60℃ for 5 min if any precipitate formd.
• Preheat dry baths or water baths to 65℃ before the operation.
• Add required ethanol (96-100%) to PG3 Buffer, GW and Wash before use.
• Store RNase A at -20℃ upon recipit of kit. Add sterile ddH2O to RNase A tube to make a 50 mg/ml stock solution. Vortex and make sure that RNase A has been completely dissolved. Store the stock solution at -20℃ .
• All centrifuge steps are done at full speed(~ 18,000 x g) in a microcentrifuge.