Blood / Cultured Cell Total RNA Mini Kit
HBBRK050 & HBBRK100
11+ Years Of Experience
Providing High Quality Test Services For Your Health!
| HBBRK050 | HBBRK100 | |
|---|---|---|
| RL Buffer | 120 ml | 240 ml |
| RB Buffer | 25 ml | 45 ml |
| Wash Buffer 1 | 30 ml | 60 ml |
| Wash Buffer 2 (concentrate) | 15 ml | 35 ml |
| RNase-free Water | 6 ml | 6 ml |
| Filter Column | 50 pcs | 100 pcs |
| RB Mini Column | 50 pcs | 100 pcs |
| Collection Tube | 100 pcs | 200 pcs |
| Elution Tube | 50 pcs | 100 pcs |
| User Manual | 1 | 1 |
Specification:
- Principle : mini spin column (silica matrix)
- Sample size : 300 μl
- Operation time : 30 ~ 60 minutes
- Binding capacity : up to 100 μg total RNA / column
- Expected yield : 1 μg
- Column applicability : centrifugation and vaccum
- Minimum elution volume : 40 μl
Important Notes:
- Make sure everything is RNase-free when handling RNA.
- Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
- Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving RB Buffer or PRB Buffer in a chemical fume hood.
- Add required volume of RNase-free ethanol (96~100%) to Wash Buffer 2 when first use.
- Dilute RNase-free DNase 1 in reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) to final conc. = 0.5 U/μl.
